| Title |
A Conserved Mechanism for Control of Human and Mouse Embryonic Stem Cell Pluripotency and Differentiation by Shp2 Tyrosine Phosphatase
|
|---|---|
| Published in |
PLOS ONE, March 2009
|
| DOI | 10.1371/journal.pone.0004914 |
| Pubmed ID | |
| Authors |
Dongmei Wu, Yuhong Pang, Yuehai Ke, Jianxiu Yu, Zhao He, Lutz Tautz, Tomas Mustelin, Sheng Ding, Ziwei Huang, Gen-Sheng Feng |
| Abstract |
Recent studies have suggested distinctive biological properties and signaling mechanisms between human and mouse embryonic stem cells (hESCs and mESCs). Herein we report that Shp2, a protein tyrosine phosphatase with two SH2 domains, has a conserved role in orchestration of intracellular signaling cascades resulting in initiation of differentiation in both hESCs and mESCs. Homozygous deletion of Shp2 in mESCs inhibited differentiation into all three germ layers, and siRNA-mediated knockdown of Shp2 expression in hESCs led to a similar phenotype of impaired differentiation. A small molecule inhibitor of Shp2 enzyme suppressed both hESC and mESC differentiation capacity. Shp2 modulates Erk, Stat3 and Smad pathways in ES cells and, in particular, Shp2 regulates BMP4-Smad pathway bi-directionally in mESCs and hESCs. These results reveal a common signaling mechanism shared by human and mouse ESCs via Shp2 modulation of overlapping and divergent pathways. |
Mendeley readers
The data shown below were compiled from readership statistics for 57 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 2% |
| Spain | 1 | 2% |
| United States | 1 | 2% |
| Australia | 1 | 2% |
| Unknown | 53 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 18 | 32% |
| Student > Ph. D. Student | 16 | 28% |
| Professor > Associate Professor | 6 | 11% |
| Student > Bachelor | 5 | 9% |
| Student > Postgraduate | 2 | 4% |
| Other | 5 | 9% |
| Unknown | 5 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 33 | 58% |
| Biochemistry, Genetics and Molecular Biology | 13 | 23% |
| Medicine and Dentistry | 3 | 5% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 4% |
| Neuroscience | 1 | 2% |
| Other | 0 | 0% |
| Unknown | 5 | 9% |