| Title |
High-Level Production of Amorpha-4,11-Diene, a Precursor of the Antimalarial Agent Artemisinin, in Escherichia coli
|
|---|---|
| Published in |
PLOS ONE, February 2009
|
| DOI | 10.1371/journal.pone.0004489 |
| Pubmed ID | |
| Authors |
Hiroko Tsuruta, Christopher J. Paddon, Diana Eng, Jacob R. Lenihan, Tizita Horning, Larry C. Anthony, Rika Regentin, Jay D. Keasling, Neil S. Renninger, Jack D. Newman |
| Abstract |
Artemisinin derivatives are the key active ingredients in Artemisinin combination therapies (ACTs), the most effective therapies available for treatment of malaria. Because the raw material is extracted from plants with long growing seasons, artemisinin is often in short supply, and fermentation would be an attractive alternative production method to supplement the plant source. Previous work showed that high levels of amorpha-4,11-diene, an artemisinin precursor, can be made in Escherichia coli using a heterologous mevalonate pathway derived from yeast (Saccharomyces cerevisiae), though the reconstructed mevalonate pathway was limited at a particular enzymatic step. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 441 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 11 | 2% |
| United Kingdom | 4 | <1% |
| France | 3 | <1% |
| Iran, Islamic Republic of | 2 | <1% |
| Germany | 1 | <1% |
| India | 1 | <1% |
| Sweden | 1 | <1% |
| Switzerland | 1 | <1% |
| Denmark | 1 | <1% |
| Other | 3 | <1% |
| Unknown | 413 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 110 | 25% |
| Researcher | 101 | 23% |
| Student > Master | 56 | 13% |
| Student > Bachelor | 34 | 8% |
| Professor | 17 | 4% |
| Other | 57 | 13% |
| Unknown | 66 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 169 | 38% |
| Biochemistry, Genetics and Molecular Biology | 90 | 20% |
| Chemistry | 41 | 9% |
| Engineering | 24 | 5% |
| Pharmacology, Toxicology and Pharmaceutical Science | 7 | 2% |
| Other | 36 | 8% |
| Unknown | 74 | 17% |