| Title |
Resurrection of a Bull by Cloning from Organs Frozen without Cryoprotectant in a −80°C Freezer for a Decade
|
|---|---|
| Published in |
PLOS ONE, January 2009
|
| DOI | 10.1371/journal.pone.0004142 |
| Pubmed ID | |
| Authors |
Yoichiro Hoshino, Noboru Hayashi, Shunji Taniguchi, Naohiko Kobayashi, Kenji Sakai, Tsuyoshi Otani, Akira Iritani, Kazuhiro Saeki |
| Abstract |
Frozen animal tissues without cryoprotectant have been thought to be inappropriate for use as a nuclear donor for somatic cell nuclear transfer (SCNT). We report the cloning of a bull using cells retrieved from testicles that had been taken from a dead animal and frozen without cryoprotectant in a -80 degrees C freezer for 10 years. We obtained live cells from defrosted pieces of the spermatic cords of frozen testicles. The cells proliferated actively in culture and were apparently normal. We transferred 16 SCNT embryos from these cells into 16 synchronized recipient animals. We obtained five pregnancies and four cloned calves developed to term. Our results indicate that complete genome sets are maintained in mammalian organs even after long-term frozen-storage without cryoprotectant, and that live clones can be produced from the recovered cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 3% |
| United Kingdom | 1 | 3% |
| Unknown | 32 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 10 | 29% |
| Student > Bachelor | 8 | 24% |
| Other | 4 | 12% |
| Student > Ph. D. Student | 4 | 12% |
| Student > Master | 1 | 3% |
| Other | 3 | 9% |
| Unknown | 4 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 16 | 47% |
| Medicine and Dentistry | 6 | 18% |
| Biochemistry, Genetics and Molecular Biology | 4 | 12% |
| Philosophy | 1 | 3% |
| Computer Science | 1 | 3% |
| Other | 3 | 9% |
| Unknown | 3 | 9% |