| Title |
Flow-Cytometric Phosphoprotein Analysis Reveals Agonist and Temporal Differences in Responses of Murine Hematopoietic Stem/Progenitor Cells
|
|---|---|
| Published in |
PLOS ONE, November 2008
|
| DOI | 10.1371/journal.pone.0003776 |
| Pubmed ID | |
| Authors |
Demetrios Kalaitzidis, Benjamin G. Neel |
| Abstract |
Hematopoietic stem cells (HSCs) are probably the best-studied adult tissue-restricted stem cells. Although methods for flow cytometric detection of phosphoproteins in hematopoeitic progenitors and mature cells are available, analogous protocols for HSC are lacking. We present a robust method to study intracellular signaling in immunophenotypically-defined murine HSC/progenitor cell (HPC)-enriched populations. Using this method, we uncover differences in the response dynamics of several phosphoproteins representative of the Ras/MAP-Kinase(K), PI3K, mTOR and Jak/STAT pathways in HSC/HPCs stimulated by Scf, Thpo, as well as several other important HSC/HPC agonists. |
Mendeley readers
The data shown below were compiled from readership statistics for 51 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | 2% |
| United States | 1 | 2% |
| Germany | 1 | 2% |
| Unknown | 48 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 22 | 43% |
| Student > Ph. D. Student | 10 | 20% |
| Professor > Associate Professor | 5 | 10% |
| Student > Master | 5 | 10% |
| Professor | 2 | 4% |
| Other | 3 | 6% |
| Unknown | 4 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 20 | 39% |
| Medicine and Dentistry | 11 | 22% |
| Biochemistry, Genetics and Molecular Biology | 9 | 18% |
| Immunology and Microbiology | 2 | 4% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 4% |
| Other | 2 | 4% |
| Unknown | 5 | 10% |