| Title |
A Francisella tularensis Schu S4 Purine Auxotroph Is Highly Attenuated in Mice but Offers Limited Protection against Homologous Intranasal Challenge
|
|---|---|
| Published in |
PLOS ONE, June 2008
|
| DOI | 10.1371/journal.pone.0002487 |
| Pubmed ID | |
| Authors |
Roger D. Pechous, Travis R. McCarthy, Nrusingh P. Mohapatra, Shilpa Soni, Renee M. Penoske, Nita H. Salzman, Dara W. Frank, John S. Gunn, Thomas C. Zahrt |
| Abstract |
Francisella tularensis is a gram-negative coccobacillus that causes the febrile illness tularemia. Subspecies that are pathogenic for humans include those comprising the type A (subspecies tularensis) or type B (subspecies holarctica) biovars. An attenuated live vaccine strain (LVS) developed from a type B isolate has previously been used to vaccinate at-risk individuals, but offers limited protection against high dose (>1000 CFUs) challenge with type A strains delivered by the respiratory route. Due to differences between type A and type B F. tularensis strains at the genetic level, it has been speculated that utilization of an attenuated type A strain as a live vaccine might offer better protection against homologous respiratory challenge compared with LVS. Here, we report the construction and characterization of an unmarked Delta purMCD mutant in the highly virulent type A strain Schu S4. |
Mendeley readers
The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 4% |
| Unknown | 24 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 32% |
| Researcher | 5 | 20% |
| Professor > Associate Professor | 3 | 12% |
| Student > Master | 2 | 8% |
| Professor | 1 | 4% |
| Other | 3 | 12% |
| Unknown | 3 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 11 | 44% |
| Immunology and Microbiology | 6 | 24% |
| Veterinary Science and Veterinary Medicine | 2 | 8% |
| Business, Management and Accounting | 1 | 4% |
| Medicine and Dentistry | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 4 | 16% |