Title |
Structural Requirements for Yersinia YopJ Inhibition of MAP Kinase Pathways
|
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Published in |
PLOS ONE, January 2008
|
DOI | 10.1371/journal.pone.0001375 |
Pubmed ID | |
Authors |
Yi-Heng Hao, Yong Wang, Dara Burdette, Sohini Mukherjee, Gladys Keitany, Elizabeth Goldsmith, Kim Orth |
Abstract |
MAPK signaling cascades are evolutionally conserved. The bacterial effector, YopJ, uses the unique activity of Ser/Thr acetylation to inhibit the activation of the MAPK kinase (MKK) and prevent activation by phosphorylation. YopJ is also able to block yeast MAPK signaling pathways using this mechanism. Based on these observations, we performed a genetic screen to isolate mutants in the yeast MKK, Pbs2, that suppress YopJ inhibition. One suppressor contains a mutation in a conserved tyrosine residue and bypasses YopJ inhibition by increasing the basal activity of Pbs2. Mutations on the hydrophobic face of the conserved G alpha-helix in the kinase domain prevent both binding and acetylation by YopJ. Corresponding mutants in human MKKs showed that they are conserved not only structurally, but also functionally. These studies reveal a conserved binding site found on the superfamily of MAPK kinases while providing insight into the molecular interactions required for YopJ inhibition. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 3% |
Germany | 1 | 3% |
Unknown | 38 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 12 | 30% |
Student > Ph. D. Student | 11 | 28% |
Student > Bachelor | 4 | 10% |
Professor | 3 | 8% |
Student > Postgraduate | 2 | 5% |
Other | 4 | 10% |
Unknown | 4 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 20 | 50% |
Biochemistry, Genetics and Molecular Biology | 7 | 18% |
Immunology and Microbiology | 2 | 5% |
Engineering | 2 | 5% |
Medicine and Dentistry | 2 | 5% |
Other | 1 | 3% |
Unknown | 6 | 15% |