Title |
Design and Pre-Clinical Evaluation of a Universal HIV-1 Vaccine
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Published in |
PLOS ONE, October 2007
|
DOI | 10.1371/journal.pone.0000984 |
Pubmed ID | |
Authors |
Sven Létourneau, Eung-Jun Im, Tumelo Mashishi, Choechoe Brereton, Anne Bridgeman, Hongbing Yang, Lucy Dorrell, Tao Dong, Bette Korber, Andrew J. McMichael, Tomáš Hanke |
Abstract |
One of the big roadblocks in development of HIV-1/AIDS vaccines is the enormous diversity of HIV-1, which could limit the value of any HIV-1 vaccine candidate currently under test. To address the HIV-1 variation, we designed a novel T cell immunogen, designated HIV(CONSV), by assembling the 14 most conserved regions of the HIV-1 proteome into one chimaeric protein. Each segment is a consensus sequence from one of the four major HIV-1 clades A, B, C and D, which alternate to ensure equal clade coverage. The gene coding for the HIV(CONSV) protein was inserted into the three most studied vaccine vectors, plasmid DNA, human adenovirus serotype 5 and modified vaccine virus Ankara (MVA), and induced HIV-1-specific T cell responses in mice. We also demonstrated that these conserved regions prime CD8(+) and CD4(+) T cell to highly conserved epitopes in humans and that these epitopes, although usually subdominant, generate memory T cells in patients during natural HIV-1 infection. Therefore, this vaccine approach provides an attractive and testable alternative for overcoming the HIV-1 variability, while focusing T cell responses on regions of the virus that are less likely to mutate and escape. Furthermore, this approach has merit in the simplicity of design and delivery, requiring only a single immunogen to provide extensive coverage of global HIV-1 population diversity. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
South Africa | 3 | 2% |
United Kingdom | 2 | 1% |
Colombia | 1 | <1% |
Germany | 1 | <1% |
Sweden | 1 | <1% |
Portugal | 1 | <1% |
France | 1 | <1% |
Spain | 1 | <1% |
Unknown | 136 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 34 | 23% |
Researcher | 33 | 22% |
Student > Bachelor | 17 | 12% |
Student > Master | 13 | 9% |
Professor | 7 | 5% |
Other | 21 | 14% |
Unknown | 22 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 42 | 29% |
Medicine and Dentistry | 40 | 27% |
Immunology and Microbiology | 14 | 10% |
Biochemistry, Genetics and Molecular Biology | 11 | 7% |
Engineering | 3 | 2% |
Other | 8 | 5% |
Unknown | 29 | 20% |