Title |
Printing Multistrain Bacterial Patterns with a Piezoelectric Inkjet Printer
|
---|---|
Published in |
PLOS ONE, July 2007
|
DOI | 10.1371/journal.pone.0000663 |
Pubmed ID | |
Authors |
Jack Merrin, Stanislas Leibler, John S. Chuang |
Abstract |
Many studies involving interacting microorganisms would benefit from simple devices able to deposit cells in precisely defined patterns. We describe an inexpensive bacterial piezoelectric inkjet printer (adapted from the design of the POSaM oligonucleotide microarrayer) that can be used to "print out" different strains of bacteria or chemicals in small droplets onto a flat surface at high resolution. The capabilities of this device are demonstrated by printing ordered arrays comprising two bacterial strains labeled with different fluorescent proteins. We also characterized several properties of this piezoelectric printer, such as the droplet volume (of the order of tens of pl), the distribution of number of cells in each droplet, and the dependence of droplet volume on printing frequency. We established the limits of the printing resolution, and determined that the printed viability of Escherichia coli exceeded 98.5%. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 20% |
India | 1 | 20% |
Netherlands | 1 | 20% |
Belgium | 1 | 20% |
Unknown | 1 | 20% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 3 | 60% |
Scientists | 2 | 40% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 6 | 5% |
France | 2 | 2% |
Italy | 1 | <1% |
Australia | 1 | <1% |
Saudi Arabia | 1 | <1% |
India | 1 | <1% |
Japan | 1 | <1% |
Denmark | 1 | <1% |
Unknown | 114 | 89% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 30 | 23% |
Researcher | 28 | 22% |
Student > Bachelor | 11 | 9% |
Other | 10 | 8% |
Professor > Associate Professor | 10 | 8% |
Other | 23 | 18% |
Unknown | 16 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 43 | 34% |
Engineering | 23 | 18% |
Physics and Astronomy | 11 | 9% |
Biochemistry, Genetics and Molecular Biology | 9 | 7% |
Chemistry | 9 | 7% |
Other | 14 | 11% |
Unknown | 19 | 15% |