@AlvaroCrevenna @DocMeeks @P_M_Mueller FLIM is nice, since it is quantitative and doesn't require a highly fluorescent acceptor (in contrast to ratiometric methods). We've seen that mCherry actually is a pretty good acceptor for FLIM: https://t.co/4kGXwMUG

@albertwcheng Another example: https://t.co/9ZR8ha53Pc And this one: https://t.co/4kGXwMUGlV

@p53p21rb @AcademicChatter Acceptor photobleaching is a low-tech alternative that should work when YFP or mCherry is the acceptor: https://t.co/scJW87zSs6

mKO photoconversion reported here: https://t.co/scJW87zSs6