Just used this construct we built in 2011 with my former PhD student @VeliaSiciliano. It works really well if you need a single plasmid to express a protein in a tetracycline-inducible way in mammalian cells. If you want it just ask me :) https://t.co/bgM

Modelling of an inducible positive feedback loop stably integrated in a Mammalian cell-line http://bit.ly/p6qUXY